Fibroblast Growth Factor-19 Human Recombinant, His Tag
Fibroblast Growth Factor -19 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 206 amino acids and having a molecular mass of 23 kDa. The amino acid sequence of the recombinant human FGF19 is 100% homologous to the amino acid sequence of the human FGF19 without signal sequence and contains his tag at N-terminal The FGF-19 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
MRGSHHHHHH GMASLAFSDA GPHVHYGWGD PIRLRHLYTS GPHGLSSCFL RIRADGVVDC ARGQSAHSLLEIKAVALRTV AIKGVHSVRY LCMGADGKMQ GLLQYSEEDC AFEEEIRPDG YNVYRSEKHR LPVSLSSAKQ RQLYKNRGFL PLSHFLPMLP MVPEEPEDLR GHLESDMFSS PLETDSMDPF GLVTGLEAVR SPSFEK.
Formulation
Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 7.5.
Physical Appearance
Filtered white lyophilized powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(c) Analysis by SDS-PAGE.
Solubility
Add deionized water to a working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by appropriate sterile filter before using it in the cell culture.
Source
Stability
Lyophilized FGF-19 Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Fibroblast Growth Factor-19 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Fibroblast growth factor 19, FGF-19.
