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Recombinant F420-dependent NADP reductase (fno)

Recombinant F420-dependent NADP reductase (fno) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520438MSQYeast1mgQuote
EP520438MSQE. coli1mgQuote
BP520438MSQBaculovirus200ugQuote
MP520438MSQMammalian Cell200ugQuote

Protein Information

SpeciesMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
UniProt IDD9PVP5
Gene Namefno; Locus:MTBMA_c06980
Protein NameF420-dependent NADP reductase
Region Expressed1-224
Expression Tag6xHis
Purity>90%
AA SequenceMKIAVLGGTGDQGLGLALRLALAGEEVIIGSRDAEKAVSAAQKVLEIAERDDLKVKGATN AEAAEEAEVAILTVPLQAQMATLGSVKEAIKGKVLIDATVPIDSCLGGSAVRYIDLWDGS AAERAARFLEDQGTRVAAAFNNISASALLDITGPVDCDCLIASDHRDALDLASELAEKID GVRAIDCGGLENARVIEKITPLLINLNIKNRIRNAGIRITNLPE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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