The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.5-2.5ng/ml.
The EPO-alpha was lyophilized from 1mg/ml in 1xPBS.
Sterile filtered white lyophilized (freeze-dried) powder.
Greater than 95% as obsereved by SDS-PAGE.
It is recommended to reconstitute the lyophilized EPO-alpha in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized EPO-alpha although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution EPO-alpha should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Erythropoietin-Alpha, EPO-a, EPO-alpha, Epoetin, EP, MGC138142.
