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Recombinant E3 UFM1-protein ligase 1 (Ufl1)

Recombinant E3 UFM1-protein ligase 1 (Ufl1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP012209RAYeast1mgQuote
EP012209RAE. coli1mgQuote
BP012209RABaculovirus200ugQuote
MP012209RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDB2GV24
Gene NameUfl1
Protein NameE3 UFM1-protein ligase 1
Region Expressed1-793
Expression Tag6xHis
Purity>90%
AA SequenceMADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPA QISKEMRDELHVRGGRVNIVDLQQVINVDLTHIENRVGDIIKSEKHVQMVLGQLVDENYL DRLSEEVNDKLQESGQVTVSELCKTYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTE AFVARHKARIRGLFSAITRPTAVNSLVSKYGFQEQLLYSVLEELVSTGRLRGTVVGGRQD KAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTPLLFLKATC VGQGLVDQVEASVEEAISSGTWVDVSPLLPSSLSVEDAAMLLQQVMRPFGKHASATVFSD TVVVSEKFINDCTKLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTNKKDKKDE RRKKATEGSGSVRGGGGGNAREYKIKKVKKKGRKDEDSDDESQSSHAGKKKPDITFMFQD EIEGCLRKHIPDAPEEFISELAEHLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQ EEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDLMMAVEDPA TITSDMRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRE RQILFQHRQALADQLKVTEDPALILHLTSVLLFQFSTHSMLHAPGRCVPQIIAFLHNKIP EDQHTLLVKYQGLVVKQLVSQNKKSGQGEDPSSDDLDKEQHDVTNTTRKELQELSLSIKD LVLKPRKSSVTEE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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