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Recombinant E3 ubiquitin-protein ligase HACE1 (Hace1)

Recombinant E3 ubiquitin-protein ligase HACE1 (Hace1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP010115RAYeast1mgQuote
EP010115RAE. coli1mgQuote
BP010115RABaculovirus200ugQuote
MP010115RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDD3ZBM7
Gene NameHace1
Protein NameE3 ubiquitin-protein ligase HACE1
Region Expressed1-909
Expression Tag6xHis
Purity>90%
AA SequenceMERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYA FGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEY SADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTV QCLLDSGADINRPNVAGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGG YGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVAT TNGHKLLSLSSSYEAQMKSLLRIVRIFCHVFRIGPSSPSNGMDMGYNGNKTPRSQVFKPL ELLWHSLDEWLVLIATELMKNKEDSADITSILLKQKGQDQEASCISAFEPPGPGSYERLS TGPGESKPDVLAGKQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFV CKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQP DSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREW FDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNI YFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEE VPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYE LELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDMTQEERVLLLQFVTGSSR VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALH CGSYGYTMA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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