The DnaK protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium phosphate buffer pH 7.4, 130mM sodium chloride and 2.5mM KCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized DnaK in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized DnaK although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution DnaK should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, 70 kDa antigen, ML2496.
