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Recombinant DNA gyrase subunit B (gyrB)

Recombinant DNA gyrase subunit B (gyrB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518857HTUYeast1mgQuote
EP518857HTUE. coli1mgQuote
BP518857HTUBaculovirus200ugQuote
MP518857HTUMammalian Cell200ugQuote

Protein Information

SpeciesHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
UniProt IDD4GZ01
Gene NamegyrB; Locus:HVO_1572
Protein NameDNA gyrase subunit B
Region Expressed1-639
Expression Tag6xHis
Purity>90%
AA SequenceMSQDNEYGAGQIQVLEGLEAVRKRPAMYIGSTDSRGLHHLVYEVVDNSIDEALAGHCDAI EVALHEDGSVSVTDNGRGIPVDTHEQYDRPALEVIMTVLHAGGKFDNKSYQVSGGLHGVG VSVVNALSSELEVEVKRDGAVWTHRFEVGEPQVEEFERVRDLEPGEDTGTTIRFWPDDGI FETTEFDFKTLENRLRELAFLNSGVEISLSDERTDESSTFLFEGGIREFVEYLNETKTAL HDDVIYYDDESEGIEVEIAMQATDELQGSIHAFANNINTREGGTHLTGFKTALTRVVNDY ANTHDMLDDLDGDNLRGEDVREGLTAVISIKHPDPQFEGQTKTKLGNSEVRGIVESVTHQ QLGTFFEENPDTATAIISKAVEAARARKAAKQAEELTRRKSALESTSLPGKLADCQSRDP AESELFIVEGDSAGGSAKQGRDRKFQAILPLKGKILNVEKHRLDRILENDEIRALITAIG GGVGDEFDIEKARYQRLILMTDADVDGAHIRTLLLTLLYRHMRPLIEAGYVYAAQPPLYR VRYRGNTYDAMDEAERDRIIEEECNGNPTQVQRFKGLGEMNPDQLWDTTMNPENRVLKRI TVEDAAAADRMFNILMGDAVGPRKQFIKDHANDAEWVDI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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