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Recombinant Diphthine synthase (dphB)

Recombinant Diphthine synthase (dphB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP007133MSPYeast1mgQuote
EP007133MSPE. coli1mgQuote
BP007133MSPBaculovirus200ugQuote
MP007133MSPMammalian Cell200ugQuote

Protein Information

SpeciesMethanosphaerula palustris (strain ATCC BAA-1556 / DSM 19958 / E1-9c)
UniProt IDB8GIF8
Gene NamedphB; Locus:Mpal_0116
Protein NameDiphthine synthase
Region Expressed1-251
Expression Tag6xHis
Purity>90%
AA SequenceMLTFVGLGLYDAGDISVKGLEAVRASDAVFLEYYTSRLMGTTIEDLVRAYGKEVIVLARA DVEQHPEPILDAAAAGDVVVLTGGDPMVSTTHMDLRLRAAARGIPTGIIHGASIQTAVCG LTGLQNYRFGKSCSVPFPQKNWFPLTPYEVVRQNLAADLHTLVYLDIQQDRYMRVGEAID LLEEMAVRVGGSITTYIGVARAGSVSPVVRAGTADHLRGIDFGGPLHVLIVPATLHPVEQ EYLEVFAGLSV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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