Request QuoteCatalog Number: xP525435DNVSize: 0.2-1mg

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Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)

Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP525435DNVYeast1mgQuote
EP525435DNVE. coli1mgQuote
BP525435DNVBaculovirus200ugQuote
MP525435DNVMammalian Cell200ugQuote

Protein Information

SpeciesAquifex aeolicus (strain VF5)
UniProt IDO67060
Gene NameispE; Locus:aq_915
Protein Name4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Region Expressed1-268
Expression Tag6xHis
Purity>90%
AA SequenceMIKVLSPAKINLGLWVLGRLPSGYHEILTLYQEIPFYDEIYIREGVLRVETNIGIPQEEN LVYKGLREFERITGIEINYSIFIQKNIPPGAGLGGGSSNLAVVLKKVNELLGSPLSEEEL RELVGSISADAPFFLLGKSAIGRGKGEVLEPVETEISGKITLVIPQVSSSTGRVYSSLRE EHFVTPEYAEEKIQRIISGEVEEIENVLGDIARELYPEINEVYRFVEYLGFKPFVSGSGS TVYFFGGASEELKKAAKMRGWKVVELEL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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