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Request QuoteCatalog Number: xP474675HXTSize: 0.2-1mg

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Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)

Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP474675HXTYeast1mgQuote
EP474675HXTE. coli1mgQuote
BP474675HXTBaculovirus200ugQuote
MP474675HXTMammalian Cell200ugQuote

Protein Information

SpeciesHydrogenobaculum sp. (strain Y04AAS1)
UniProt IDB4U5Q9
Gene NameispE; Locus:HY04AAS1_1414
Protein Name
Region Expressed1-284
Expression Tag6xHis
Purity>90%
AA SequenceMNKKNNLLVLEDGVKVILAPAKVNFGLWIKGKRPDGYHDIFSIIHTIDLYDRIYIELHYT LEVLSVGPFSKNLKDNIVYEGVLAFSRLTGKNFDYKIVIEKNIPVGAGLGGASSDLAAVI SYLNEELENPMKEQELTDFLSSFSKDAPFFLKGGCALVYGTGDQVKVLEPISREITVVYP NIEASTSKVYGAFTKQTEEVLLLEDILRLLEENDIENIIENHLQETAIEIYKEIGELIRF LESVGYKPYMSGSGSSVYVFGKLSDKIKMALESRGWYVYECKTI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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