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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP405492BOPYeast1mgQuote
EP405492BOPE. coli1mgQuote
BP405492BOPBaculovirus200ugQuote
MP405492BOPMammalian Cell200ugQuote

Protein Information

SpeciesBacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154)
UniProt IDA6KZJ2
Gene NamemiaB; Locus:BVU_1165
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-456
Expression Tag6xHis
Purity>90%
AA SequenceMKETTGADFKSATDSENKKLFIETYGCQMNVADSEVIASIMQMAGYHVCETLDEADAVFM NTCSIRDNAEQKILNRLEFFHSMKKNKRRNLIVGVLGCMAERVKDDLIENHHVDLVVGPD AYLTLPDLVASVEAGEKAINVELSTTETYREVIPSRICGNHISGFVSIMRGCNNFCHYCI VPYTRGRERSRDVESILNEVRDLADKGYKEVTLLGQNVNSYRFEKEGEIITFPMLLRTVA EAVPDMRVRFTTSHPKDMSDETLQVIAETPNVCKHIHLPVQSGSSRILKLMNRKYTREWY LERVAAIRRIIPDCGLSTDIFSGYHSETEEDHQESLSLMRECAYDSAFMFKYSERPGTYA SKHLPDDVPEEVKIRRLNEIIELQNRLSAESNARDVGKTFEVMVEGVSKRSREQLFGRTQ QNKVVVFDRGNHRIGDFVHVRITEASSATLKGEEVF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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