Request QuoteCatalog Number: xP406025PZRSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP406025PZRYeast1mgQuote
EP406025PZRE. coli1mgQuote
BP406025PZRBaculovirus200ugQuote
MP406025PZRMammalian Cell200ugQuote

Protein Information

SpeciesPsychrobacter sp. (strain PRwf-1)
UniProt IDA5WGM4
Gene NamemiaB; Locus:PsycPRwf_1875
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-490
Expression Tag6xHis
Purity>90%
AA SequenceMSATLFDPKSIAVSNPVDTSTQASLAHASQPTGQAKKKVYIATQGCQMNVYDSEKMGNVL GDSHDMVVTDNIEEADVLLMNTCSIREKAQEKVFSELGRWRKLKEEKPDLVIGVGGCVAS QEGDNIQKRAPYVDMVFGPQTLHRLPELYDKSTTQRNVKPKDRIGTVDVSFPSIEKFDFL PEPKVEGYRAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAEQGVREITLL GQNVNGYRGEKDDGSICRFAELLHYVAHVDGIERIRYTTSHPLEFTDDIIEAYAKLPQLV SHLHLPVQSGSNKILAAMKRNHTIDVYINQINKLMAVRPDMHLSSDFIIGFPGETEEDFL DTLNLAKALDFDHSYSFIYSKRPGTPASELPDDVSLATKKERLAIFQKVIRDSTLKKTEE MVGKTLRVMVEEIADRYPDQLLGTADNTRSVLFKATEQQKTDLMGKFVTVKITDFVSPHM VRGELVDILD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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