Request QuoteCatalog Number: xP395443RLGSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP395443RLGYeast1mgQuote
EP395443RLGE. coli1mgQuote
BP395443RLGBaculovirus200ugQuote
MP395443RLGMammalian Cell200ugQuote

Protein Information

SpeciesRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
UniProt IDA4WZB3
Gene NamemiaB; Locus:Rsph17025_3872
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMSESRKLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKV YSDLGRLRPLKVARPDLKIGVAGCVAQAEGEEILRRMPLVDLVVGPQSYHRLPDMLERTE GGARVIDTDFPEEDKFEHLPERKALRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFA RLMAEARALVEKGVREITLLGQNVNAWSNDGRGLGGLIRELARIDGLERLRYTTSHPNDM ADDLIEAHGQEPKLMPYLHLPVQSGSDRILKAMNRKHAAEQYLRLVERIRAARPDILLTS DFIVGFPGETDADFEATLALIRAVGFGSAFSFKYSARPGTPAAEKPELPAELCDARLQTL QALLGEQQRAAQAAMVGRELGVLYEKAGRLPGQMVGKSDYLHAVHVEDPGARPGDLVRVR ITASGPNSLAGERIGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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