Request QuoteCatalog Number: xP388572MOLSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388572MOLYeast1mgQuote
EP388572MOLE. coli1mgQuote
BP388572MOLBaculovirus200ugQuote
MP388572MOLMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium sp. (strain JLS)
UniProt IDA3PYF3
Gene NamemiaB; Locus:Mjls_2144
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-526
Expression Tag6xHis
Purity>90%
AA SequenceMTQQAANALPPVARTYQVRTYGCQMNVHDSERLAGLLEDAGYRRAADGADADVVVFNTCA VRENADNKLYGNLSHLAPRKRSEPQMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGS LPTLLERARHNRAAQVEIAEALQEFPSTLPAARESAYAGWVSISVGCNNTCTFCIVPSLR GKEVDRRPGDVLAEIQTLVDQGVLEVTLLGQNVNAYGVSFAADERLREDPRMWQSAPHRN RGAFAELLRACGRIDGLERVRFTSPHPAEFTDDVIEAMAETPNVCPALHMPLQSGSDRIL RAMRRSYRAEKYLGIIDRVRAAIPDAAITTDLIVGFPGETEEDFQATLDVVAASRFSSAF TFQYSKRPGTPAADMPGQLPKAVVSERYQRLIELQERISLEENQAQVGRTLELLVATGEG RKDAATARLSGRARDGRLVHFAPGAAADEPLARRAFDQVRPGDVVTTTVTGAAPHHLIAD GALLTHRRTRAGDAHAAGLRPRTGVGLGIPGVGAPAPAPVTTGCAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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