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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP404163SUFYeast1mgQuote
EP404163SUFE. coli1mgQuote
BP404163SUFBaculovirus200ugQuote
MP404163SUFMammalian Cell200ugQuote

Protein Information

SpeciesSphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
UniProt IDA5V8Y0
Gene NamemiaB; Locus:Swit_2387
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-453
Expression Tag6xHis
Purity>90%
AA SequenceMGRGMSRPAPKSFHVKSFGCQMNVYDGARMAELLEAQGMHAADSADAADLVVLNTCHIRE KAAEKVYSDIGRIVKKAADAEEGRARPMIAVAGCVAQAEGPEIPRRAPAVDIVVGPQAYH NLPQLVADAAEGRRALDTDMPAASKFDTLPKRRRQGPTAFLTVQEGCDKFCTYCVVPYTR GAEISRPWGAIVDEAKALVDAGAREITLLGQNVNAWTGEDDRGRTQGLDGLIRALDALPG LARIRYTTSHPNDMSDGLIAAHGEVAKLMPFLHLPVQAGSDRILKAMNRSHDAAGYLRLI ERVRAARPDIAVSGDFIVGFPGETDEDFEATLAIVRAVDHAQAFSFKYSPRPGTPAASMD GQVAPAVMDERLQRLQALLNEQQHRFNLATVGKRCEVLIERDGKKPGQRIGKSPWLQSVI VEDGPAIGTLVTVDIVSAGPNSLSGALVEQKAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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