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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP412375NFAYeast1mgQuote
EP412375NFAE. coli1mgQuote
BP412375NFABaculovirus200ugQuote
MP412375NFAMammalian Cell200ugQuote

Protein Information

SpeciesNitratiruptor sp. (strain SB155-2)
UniProt IDA6Q531
Gene NamemiaB; Locus:NIS_1483
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-433
Expression Tag6xHis
Purity>90%
AA SequenceMKKRLYIETLGCAMNVRDSEHIIAELTQKEDYELTQNLQEADLILINTCSVREKPVHKLF SEIGYFNKKKKEGAKIGVCGCTASHLGEEIIKKAPYVSFVLGARNVSKIRDVIKQEKAVE VDIDYDESTYAFSDFRTSPYKAFINISIGCDKKCTFCIVPNTRGEEISIPPELILQEVRR AVDTGAKEIFLLGQNVNNYGRSFSDKARKVDFTDLLRMVSEIDGVRRIRFTSPHPLHMDD KFLQEFATNPKICKSMHMPLQSGSTKVLRDMKRGYTKEWFLDRAMKLREMVPEVHISTDI IVGFPGESEEDFADTIDVVQKVRFEQIFSFKYSPRPLTPAKDYENQIPDEVASRRLSYLQ DLHLQMLDSISEQEKDKVYEVYFEELKPGGYVAGRTDNNWIVKVKGSEELLGEFKSVRIT KPGRLSLEGELVG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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