Request QuoteCatalog Number: xP375391MOJSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP375391MOJYeast1mgQuote
EP375391MOJE. coli1mgQuote
BP375391MOJBaculovirus200ugQuote
MP375391MOJMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium bovis (strain BCG / Pasteur 1173P2)
UniProt IDA1KM71
Gene NamemiaB; Locus:BCG_2746c
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMVAHDAAAGVTGEGAGPPVRRAPARTYQVRTYGCQMNVHDSERLAGLLEAAGYRRATDGS EADVVVFNTCAVRENADNRLYGNLSHLAPRKRANPDMQIAVGGCLAQKDRDAVLRRAPWV DVVFGTHNIGSLPTLLERARHNKVAQVEIAEALQQFPSSLPSSRESAYAAWVSISVGCNN SCTFCIVPSLRGREVDRSPADILAEVRSLVNDGVLEVTLLGQNVNAYGVSFADPALPRNR GAFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTRNVCPALHMPLQSGSDRILR AMRRSYRAERYLGIIERVRAAIPHAAITTDLIVGFPGETEEDFAATLDVVRRARFAAAFT FQYSKRPGTPAAQLDGQLPKAVVQERYERLIALQEQISLEANRALVGQAVEVLVATGEGR KDTVTARMSGRARDGRLVHFTAGQPRVRPGDVITTKVTEAAPHHLIADAGVLTHRRTRAG DAHTAGQPGRAVGLGMPGVGLPVSAAKPGGCR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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