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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP399720VDYYeast1mgQuote
EP399720VDYE. coli1mgQuote
BP399720VDYBaculovirus200ugQuote
MP399720VDYMammalian Cell200ugQuote

Protein Information

SpeciesVesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA)
UniProt IDA5CW77
Gene NamemiaB; Locus:COSY_0688
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMINKLYIRTFGCQMNEYDSNKMVDILKHSHDLTLTDDATSADVLLLNTCSIREKAQDKLF HQLGRWSKLKNKNPNLIIGVGGCVASQEGELILKRTPYVDIIFGPQTLHRLPNMLNDALN NKKTSIDISFPEIEKFDHLPKPRTNSVTAFVSIMEGCSKYCTFCIVPYTRGEEISRPFND VINEVKILASQGVREINLLGQNVNAYQGSMNDGEVSDLALLINIVAKISGIKRIRYTTSH PNQFSDSLIEAYAEVPELVSHLHLPVQSGSDKILRLMKRGYMSIEYKSKIRKLRKIRPDI SISSDFIIGFPGENEKDFLDTMILIDEIGFDKSFSFIYSVRPGTPAASYPDDIDIQVKKQ RLALIQKTINNNTEHISQLMIGSIQKVLVENITKKGNELFGRTENMRNTHFKGDKSLIGQ IVNVKITKGRGNSLMGDLLTQI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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