Request QuoteCatalog Number: xP397314CWTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP397314CWTYeast1mgQuote
EP397314CWTE. coli1mgQuote
BP397314CWTBaculovirus200ugQuote
MP397314CWTMammalian Cell200ugQuote

Protein Information

SpeciesClavibacter michiganensis subsp. michiganensis (strain NCPPB 382)
UniProt IDA5CSL5
Gene NamemiaB; Locus:CMM_2023
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-528
Expression Tag6xHis
Purity>90%
AA SequenceMSTLAEHVRDTPPAAERPRTYEVRTYGCQMNVHDSERLTGSLEAAGYVSAEGAEADIVVI NTCAVRENADNKLYGNLGHLAGVKRRHEGMQIAVGGCLAQKDRATVLEKAPWVDVVFGTH NMGALPTLLERARHNGEAQLEILESLETFPSTLPTKRDEIASGWVSISVGCNNTCTFCIV PALRGKEKDRRPGDILAEIQALVDDGAVEVTLLGQNVNSYGVEFGDRQAFGKLLRAAGAI EGLERIRFTSPHPAAFTDDVIDAMAETPAVMPQLHMPLQSGSDRVLKAMRRSYRSERFLG ILDRVRTRIPDAAITTDIIVGFPGETEEDFQETLRVVEAARFSSAFTFQYSIRPGTPAAT MEEQVPADVVKERYGRLTALQERISHEENQRVVGRTVEVLVSAHEGRKDGDTRRVTGRAQ DGRLVHLDVPAGSGEPRPGDAVEVEITRAAPFHLIADSVDQAPLRIRRTRAGDAWERAQA DSCGVPAPAAGTSAGGPPRVSLGLPSLRASTIASAGAVDGSAHPRHRA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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