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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP409165KAXYeast1mgQuote
EP409165KAXE. coli1mgQuote
BP409165KAXBaculovirus200ugQuote
MP409165KAXMammalian Cell200ugQuote

Protein Information

SpeciesKlebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)
UniProt IDA6T6B5
Gene NamemiaB; Locus:KPN78578_06750; ORFs:KPN_00686
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVF HQLGRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHYVDIIFGPQTLHRLPEMINSVRG NRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPCDD ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARPDI QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEADKKQ RLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKNIMELSGRTENNRVVNFEGTPDLVG KFVDVEIVDVYTNSLRGKIVRTEAEMGLRIAESPESVIARTRKENDLGVGIYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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