Request QuoteCatalog Number: xP413169FLGSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP413169FLGYeast1mgQuote
EP413169FLGE. coli1mgQuote
BP413169FLGBaculovirus200ugQuote
MP413169FLGMammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus aureus (strain Newman)
UniProt IDA6QGJ1
Gene NamemiaB; Locus:NWMN_1201
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-514
Expression Tag6xHis
Purity>90%
AA SequenceMNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEK YRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAE NKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEI LEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRF TTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDR IPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLN VKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK EMIGKLVEVRIDEAKQYSLNGSFIKEVEPEMVIQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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