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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP376763AUZYeast1mgQuote
EP376763AUZE. coli1mgQuote
BP376763AUZBaculovirus200ugQuote
MP376763AUZMammalian Cell200ugQuote

Protein Information

SpeciesArthrobacter aurescens (strain TC1)
UniProt IDA1R550
Gene NamemiaB; Locus:AAur_1596
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMSLTIPSPIAATTPSAASMTAGEPKTYQVRTFGCQMNVHDSERMAGLLEEAGYVPADGEV ADVVVFNTCAVRENADNKLYGNLGQLRQVKEANPGMQIAVGGCLAQKDRETIVKKAPWVD AVFGTHNVGALPALLNRARHNNEAQLEILESLDVFPSTLPTKRDSVYAGWVSISVGCNNT CTFCIVPSLRGKEKDRRPGEILAEIQALVDDGAVEVTLLGQNVNSYGVEFGDRQAFSKLL RACGEIEGLERVRFTSPHPAAFTDDVIDAMAETHNAMPQLHMPLQSGSDKVLKDMRRSYR SSKFLGILDKVRDRIPHAAITTDIIVGFPGETEEDFQATLDVVEKSRFASAFTFQYSKRP GTPAADLPEQLPKAVVQERYERLTALQDRIAAEENAKQLGRKVEVLVTAQSGRKAGETHR LSGRSKDQRLVHFSVPAGAEAPRPGDFVTVTITEAAAFHLVADPAGADDYLLRRSRAGDA WDRSQADSCGVPAAGAASGKAGVSLGMPSLPTRRA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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