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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP399251BPEYeast1mgQuote
EP399251BPEE. coli1mgQuote
BP399251BPEBaculovirus200ugQuote
MP399251BPEMammalian Cell200ugQuote

Protein Information

SpeciesBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
UniProt IDA5E855
Gene NamemiaB; Locus:BBta_0046
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMTPPRKLHIKSYGCQMNVYDAQRMVDTLGAEGFVETAEAGDADLVILNTCHIREKASEKV YSELGRLRVAKEEAARQGRAMQIAVAGCVAQAEGAEIVTRAPTVDVVVGPQSYHHLPELL ARAGRGEPAIETEFPAEDKFGFLARPSREAIRARGVSAFVTVQEGCDKFCTFCVVPYTRG SEMSRPVARILDEVTRLTENGVREITLIGQNVNAYHGEAPDGSTWTLGRLLYGIAEIPGV ARIRYSTSHPNDVDDSLIAAHRDLASVMPFVHLPVQSGSDRILGLMNRKHGASDYRKVVD RFRAARPDIAFSSDFIVGFPGETEEDFRATLALIAQIGYAAAYSFKYSPRPGTPAADMQE MVSATEMDERLERLQSLIDSQQAAFNKAAIGTVVDVLFERAARKPGQLVGRTAYLQPAHV MAPDNIIGQVLPVRIDSLERYSLLGELVATAAPVAGAATPLVSIGG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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