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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP367027AVAYeast1mgQuote
EP367027AVAE. coli1mgQuote
BP367027AVABaculovirus200ugQuote
MP367027AVAMammalian Cell200ugQuote

Protein Information

SpeciesArthrobacter sp. (strain FB24)
UniProt IDA0JUY6
Gene NamemiaB; Locus:Arth_1462
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-522
Expression Tag6xHis
Purity>90%
AA SequenceMSLTIPSPASGTSTSATTDTAPAAAPQRTYQVRTFGCQMNVHDSERMAGMLEDAGYVPAS GENADVVVFNTCAVRENADNKLYGNLGMLAPVKAANPGMQIAVGGCLAQKDRETILKKAP WVDAVFGTHNVGALPALLDRARHNNEAQLEILESLDVFPSTLPTKRDSVYSGWVSISVGC NNTCTFCIVPALRGKEKDRRPGDILAEIQALVDDGAIEVTLLGQNVNSYGVEFGDRQAFS KLLRACGEIQGLERVRFTSPHPAAFTDDVIDAMAETPNVMPQLHMPLQSGSDKVLKDMKR SYRSTKFLGILDKVRERIPHAAISTDIIVGFPGETEEDFQATLDVVEKSRFATAFTFQYS KRPGTPAADLPDQLPKAVVQERFERLTALQDRIAAEENARQLGRRVEVMVTAQSGRKSEE THRLSGRSQDQRLVHFSVPEGAEKPRPGDLVTVTITEAAAFHLVADPASAADYSLRRSRA GDAWDRSQADSCGAPVAGGGAGSNGGKGGVSLGMPALPVRRS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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