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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP879204XBLYeast1mgQuote
EP879204XBLE. coli1mgQuote
BP879204XBLBaculovirus200ugQuote
MP879204XBLMammalian Cell200ugQuote

Protein Information

SpeciesXylella fastidiosa
UniProt IDQ9PEX2
Gene NamemiaB; Locus:XF_0906
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-497
Expression Tag6xHis
Purity>90%
AA SequenceMTGTSNIPTHGKEHKDAPALLPLPAPNPHHTHAAHPGNPSHDRPPSRGKLFIKTHGCQMN EYDSAKMADVLTTTEALELTDNPEEADIILINTCSIREKAQEKVFSQLGRWRALKTSGRD VIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPDMIRARREQNRPQVDIRFPEIEK FDHLPTPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLTEIAHLATQGVRE INLLGQNVNAYRGAMDPGPSNNTNPAPPPYADLGLLIRAIAQFESIGRIRFTTSHPLEFS DSLVEAYRDVPQLANHLHLPVQSGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSD FIIGFPGESDTDFQKTMQLIEDIGFDQSFSFIYSRRPGTPASNLEDHTPDEIKRTRLEHL QKHINTYAADISKRMIGTVQTVLVEGPSKKNPNELTGKTENMRPVNFPGHPRLIGQFIDV HITEALSNSLRGRVHTN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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