Request QuoteCatalog Number: xP853637DUBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP853637DUBYeast1mgQuote
EP853637DUBE. coli1mgQuote
BP853637DUBBaculovirus200ugQuote
MP853637DUBMammalian Cell200ugQuote

Protein Information

SpeciesClostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
UniProt IDQ97I18
Gene NamemiaB; Locus:CA_C1838
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-441
Expression Tag6xHis
Purity>90%
AA SequenceMDKNKLFFIETWGCQMNAEDSEKLAGMLKEMKYEATDNREDADLIIFNTCCVRENAELKV YGNLGTLKKLKDKKPNLIITVCGCMMQQRDMAEHIKKRFPFVDIVMGTHNTQMFPQYLKK VENERTSVVEIWDKEEGIVEGMPIYRSYDMKAFVTIMYGCNNFCTYCIVPYVRGRERSRK PEDIENEIKELVKKGYKEITLLGQNVNSYGKSLDEEMNFALLLRRLNNIEGLLRIRFMTS HPKDLTDDVIAAIADCDKVCEHIHLPVQSGSTTILNKMNRNYTREDYINLVNKIKSGIKN VAITTDIIVGFPGETEEDFEDTLSLVKEVEYDSAFTFLYSIREGTPAAKYENQIPDEVKH KRFNKLLEAVNLISEKKNKEYEGKIVEILVEGKSKNDGSKLMGRTRTGKLVNFEGLESSI GKLINVKITKAQAFSLVGEEV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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