Request QuoteCatalog Number: xP867829VEXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP867829VEXYeast1mgQuote
EP867829VEXE. coli1mgQuote
BP867829VEXBaculovirus200ugQuote
MP867829VEXMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
UniProt IDQ9KTE0
Gene NamemiaB; Locus:VC_0962
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKKPGVVIGVGGCVATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQT SDAPVMDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD VLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTTSH PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI QISSDFIVGFPGETDKDFQDTMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVKKE RLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMELRGRTENNRVVNFEGSPDLIG QFVDVKIVDVFPNSLRGELLRTEQEMNLRIATSPSEMKAKTRREDELGVATFTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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