Request QuoteCatalog Number: xP838100RARSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP838100RARYeast1mgQuote
EP838100RARE. coli1mgQuote
BP838100RARBaculovirus200ugQuote
MP838100RARMammalian Cell200ugQuote

Protein Information

SpeciesRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
UniProt IDQ8Y206
Gene NamemiaB; Locus:RSc0531
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMKKVFIKTFGCQMNEYDSDKMADVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFS DLGRVKALKARNPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPELIDARRRT GRPQVDVSFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV LAEVAGLAEQGVREVTLLGQNVNAYIGKMGGTSERADFALLLEYVAEIPGIERIRYTTSH PKEFTARLIEAYATNRKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSIIRKLRAIRPDI SIATDFIVGFPGETDADFAKTMDLVHEIGYDNSFSFIYSPRPGTPAANLHDDTPHAVKLE RLKHLQATIDANMARISEGMVGSVQRILVEGPSRKDPSELHGRTENNRVVNFALPDLPQA RRDQLVGQMLDVRIVHAYPHSLRGDVVEQRPDGVTQH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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