Request QuoteCatalog Number: xP819244TCXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP819244TCXYeast1mgQuote
EP819244TCXE. coli1mgQuote
BP819244TCXBaculovirus200ugQuote
MP819244TCXMammalian Cell200ugQuote

Protein Information

SpeciesThermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Caldanaerobacter subterraneus subsp. tengcongensis)
UniProt IDQ8RA72
Gene NamemiaB; Locus:TTE1356
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMRNSYYVSEEELKKQREIMEEIAWENRGKEVYYHIETYGCQMNVHDSEKLAGMLEEMGYK YTEDLEKADVLLFNTCAVREHAEVRVLGRVSQIKELKNRNPNLIIGISGCMMQEKHIVEA IREKYPHVDIVFGTHNIYKFPELLWQALNSRVQVIDVIENTQNVIEGLPIRRDSNLKAWV NIIYGCNNFCTYCIVPYTRGREKSRRPEDIIAEVKELAEKGYKEITLLGQNVNSYGKDLG GDINFAKLLYMLNDIKGIERIRFMTSHPKDISDELIYAMRDLDKVCEHLHLPVQAGSNKI LKRMNRRYTREHYLEIIDKVRANIPDIAITTDIIVGFPGETEEDFFQTLDLVERVRFDGA YTFMYSKRAGTPAASMPDQVDDEVKKDRLIRLIELQNKISLEKSAELKGKVVEVLIEGVS KRDPNKLTSRTRTNKVVHFIGDESLIGKLANVKITETKAWTMQGELVEVLR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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