Request QuoteCatalog Number: xP820574NHRSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP820574NHRYeast1mgQuote
EP820574NHRE. coli1mgQuote
BP820574NHRBaculovirus200ugQuote
MP820574NHRMammalian Cell200ugQuote

Protein Information

SpeciesNostoc sp. (strain PCC 7120 / UTEX 2576)
UniProt IDQ8YXA3
Gene NamemiaB; Locus:alr1312
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-454
Expression Tag6xHis
Purity>90%
AA SequenceMTTSNRRYHITTFGCQMNKADSERMAGILEDMGFKFSEDPNNADLILYNTCTIRDNAEQK VYSYLGRQAKRKHEQPDLTLVVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESV FAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPAA VRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGVERIR FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRR YMPDASISADAIVGFPGETEEQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSE EVKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKDQTQVMGRTGGNRLTFFKGD IHELKGQLVMVKINEVRAFSLTGEPIEMRQALPI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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