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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP837239FDQYeast1mgQuote
EP837239FDQE. coli1mgQuote
BP837239FDQBaculovirus200ugQuote
MP837239FDQMammalian Cell200ugQuote

Protein Information

SpeciesFusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
UniProt IDQ8RG43
Gene NamemiaB; Locus:FN0475
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-435
Expression Tag6xHis
Purity>90%
AA SequenceMKKASIITYGCQMNVNESAKIKKIFQNLGYDVTEEIDNADAVFLNTCTVREGAATQIFGK LGELKALKEKRGTIIGVTGCFAQEQGEELVKKFPIIDIVMGNQNIGRIPQAIEKIENNES THEVYTDNEDELPPRLDAEFGSDQTASISITYGCNNFCTFCIVPYVRGRERSVPLEEIVK DVEQYVKKGAKEIVLLGQNVNSYGKDFKNGDNFAKLLDEICKVEGDYIVRFVSPHPRDFT DDVIEVIAKNKKISKCLHLPLQSGSSQILKKMRRGYTKEKYLALVDKIKSKIPGVALTAD IIVGFPGETEEDFLDTIDVVQKVSFDNSYMFMYSIRKGTKAATMDNQIEESVKKERLQRL MEVQNKCSFYESSKYKGRIVKVLVEGPSKKNKEVLSGRTSTNKIVLFRGNLALKGQFINV KINECKTWTLYGEIV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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