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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP844315XADYeast1mgQuote
EP844315XADE. coli1mgQuote
BP844315XADBaculovirus200ugQuote
MP844315XADMammalian Cell200ugQuote

Protein Information

SpeciesXanthomonas axonopodis pv. citri (strain 306)
UniProt IDQ8PJR9
Gene NamemiaB; Locus:XAC2461
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-484
Expression Tag6xHis
Purity>90%
AA SequenceMPGTPVSDLSAATAVDATALLPLPVARPSAPAMVRGKLYIKTHGCQMNEYDSAKMADVLA ASEGLELTDDPEEADVVLVNTCSIREKAQEKVFSQLGRWKALKAGGKPVIIGVGGCVASQ EGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGKSQVDISFPEIEKFDRLPEPRAEGP SAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDVLVEVAQLAAQGVREINLLGQNVNAYR GAYGADAGDAAQYADLGLLIRTIAQIEGIGRIRFTTSHPLEFSDSLVDAYRDVPQLANYL HLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAVRPDISISSDFIVGFPGETEADFEKTM KLIEDVGFDQSFSFVYSRRPGTPASDLQDDTPEAVKQARLARLQAHINAHAASISQSMVG SVQRVLVEGPSRRDPNELTGKSENMRPVNFPGNPRLIGQFVDVLITEAMSNSLRGRIQLD DSAH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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