Request QuoteCatalog Number: xP820339CMBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP820339CMBYeast1mgQuote
EP820339CMBE. coli1mgQuote
BP820339CMBBaculovirus200ugQuote
MP820339CMBMammalian Cell200ugQuote

Protein Information

SpeciesClostridium perfringens
UniProt IDQ8XLE9
Gene NamemiaB; Locus:CPE1093
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMTLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVREN AENKVFGNLGQLKQLKKKNPNLVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFP EYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVTIMYGCNNFCTYCIVPYVRGR ERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERV RFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIK SEIPDVTLTTDIIIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIP DDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAG DEKLVGELVNLKIVRAQPFSLIGEIVE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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