Request QuoteCatalog Number: xP802588NHHSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP802588NHHYeast1mgQuote
EP802588NHHE. coli1mgQuote
BP802588NHHBaculovirus200ugQuote
MP802588NHHMammalian Cell200ugQuote

Protein Information

SpeciesNitrosomonas europaea (strain ATCC 19718 / NBRC 14298)
UniProt IDQ82SI7
Gene NamemiaB; Locus:NE2335
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-443
Expression Tag6xHis
Purity>90%
AA SequenceMGSKLYIRTFGCQMNEYDSAKMADILLSEKGMELAETPEEADLILFNTCSVREKAQEKVF HDLGRVRHLKNSKPDLLIGVGGCVASQEGPEIVKRAPFVDLVFGPQTLHRLPDLIDARRR TGRPQVDISFPEIEKFDRLPPARTEGSTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD VLTEVAGLAIQGVKEVTLLGQNVNAYLGKMINGEIADFATLLDYIHEIPGIERIRYTTSH PREFTARLIEAYQRLPKLVGHVHLPVQSGSDRILAAMKRGYTTVEYKSIVRKLRLVRPDI SISSDFIIGFPGETEDDFEATMKLIDDVHFDESFSFIYSPRPGTPAADLPDNTSHQIKLT RLYRLQEKIQLNAQAISQGMVDTVQRILVEGPSRKDPGEFCGRTDNNRVVNFAGHAGLTG SFIDIRITAVSSHTLRGEISDMQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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