Request QuoteCatalog Number: xP772491SNNSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP772491SNNYeast1mgQuote
EP772491SNNE. coli1mgQuote
BP772491SNNBaculovirus200ugQuote
MP772491SNNMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces avermitilis
UniProt IDQ82KC4
Gene NamemiaB; Locus:SAV_2479
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-508
Expression Tag6xHis
Purity>90%
AA SequenceMSSIDRSQPVGVKTYEVRTYGCQMNVHDSERLSGLLEDAGYVRAPDGSDGDADVVVFNTC AVRENADNRLYGNLGRLAPMKTKRPGMQIAVGGCLAQKDRDTIVTKAPWVDVVFGTHNIG KLPVLLERARVQEEAQVEIAESLEAFPSTLPTRRESAYAAWVSISVGCNNTCTFCIVPAL RGKEKDRRTGDILAEIEALVGEGVSEITLLGQNVNAYGSDIGDREAFSKLLRACGQIDGL ERVRFTSPHPRDFTDDVIAAMAETPNVMPQLHMPMQSGSDTVLKAMRRSYRQDRYLGIIE KVRAAIPHAAITTDIIVGFPGETEEDFEQTMHAVREARFTQAFTFQYSKRPGTPAATMEN QIPKEVVQARYERLVALQEEISWDENKKQVGRTLELMVAEGEGRKDGATHRLSGRAPDNR LVHFTKPDEEVRPGDVVTVEVTYAAPHHLLAEGPVLNVRRTRAGDAWEKRHIEKTAEAAK PAGVMLGLPKIGAPEPLPVATGSGCGCD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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