Request QuoteCatalog Number: xP812601CQGSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP812601CQGYeast1mgQuote
EP812601CQGE. coli1mgQuote
BP812601CQGBaculovirus200ugQuote
MP812601CQGMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium efficiens
UniProt IDQ8FPD5
Gene NamemiaB; Locus:CE1843
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-524
Expression Tag6xHis
Purity>90%
AA SequenceMNTHPSHPDHPADTLPARGNREGTTARTYEVRTFGCQMNVHDSERLSGLLEEAGYTAAGE GDTPDLIVFNTCAVRENADQRLYGTLGNLRAVKENHPGMQIAVGGCLAQKDKDTVVKKAP WVDVVFGTHNIASLPTLLNRAEHNQKAEVEIVDSLEQFPSVLPAKRESAYAGWVSVSVGC NNTCTFCIVPSLRGKEQDRRPGDILAEVQALVDQGVSEVTLLGQNVNAYGVNFVDPELER DRSAFSKLLRACGDIEGLERVRFTSPHPAEFTSDVIDAMAETPNICPQLHMPLQSGSDRV LKEMRRSYRSAKFLAILDEVRAKIPHASITTDIIVGFPGETEEDFQATLDVVEKARFTSA YTFQYSPRPGTPAADYENQVPKKVVQERYERLMALQERICEEENQKFIGQTVELLVQAGG GRKNDATKRMSGRARDGRLVHFTPVGTIDGEIRPGDVVTVEVTEAKPFFLIADAGVLTHR RTRAGDNSAVGQVPTTAPIGVGLGLPRIGAPVPAPATPDNACGC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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