Request QuoteCatalog Number: xP816427OABSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP816427OABYeast1mgQuote
EP816427OABE. coli1mgQuote
BP816427OABBaculovirus200ugQuote
MP816427OABMammalian Cell200ugQuote

Protein Information

SpeciesOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
UniProt IDQ8EQR4
Gene NamemiaB; Locus:OB1628
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-519
Expression Tag6xHis
Purity>90%
AA SequenceMNEQQRKQQSQIRTEQANVDRIKNTSSEDMIAKYFQQEYEPQSPDIKQARKRKREDVQFH YDFSIPEDMEKIGHGKKFLIRTYGCQMNEHDTEVMAGILSEMGYESTTVTEEADIILLNT CAIRENAENKVFGEIGHLKPLKLENPDLIIGVCGCMSQEESVVDRILQKHQHIDLIFGTH NIHRLPHLVKEALFGKEMIVEVWSKEGDIIENLPKARKGKIKAWVNIMYGCDKFCTYCIV PMTRGKERSRRPKDIIQEVRHLVAQGYQEVTLLGQNVNAYGKDFEDIEYGLGDLMNDIHK IDIPRVRFTTSHPRDFDDRLIEVLAQGGNLLDHIHLPVQSGSSEILKKMNRKYTREEYLE LVRKIRIAMPNATLTTDIIVGFPNETEEQFEETMTLVEEVGFEAAYTFIYSPREGTPAAR KKDDVPEEVKKQRLYRLNELVNKQSAASMKDYAGKKVKVLVEGESKKDPEVLAGYTEKNK LVNFKGPKSSIGKIVEVEITETKTWSLNGVMVENTVEVN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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