Request QuoteCatalog Number: xP818054BGPSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP818054BGPYeast1mgQuote
EP818054BGPE. coli1mgQuote
BP818054BGPBaculovirus200ugQuote
MP818054BGPMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium longum (strain NCC 2705)
UniProt IDQ8G4H4
Gene NamemiaB; Locus:BL1408
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-480
Expression Tag6xHis
Purity>90%
AA SequenceMMTEAERASIAADGTDGLLGKRGKGVFHIHTLGCQMNVHDSERIAGVLEANGYVPATEDQ INDNDLDLLVLNTCAVRENAAERMYGTIGRFNRVKLVRPNLQIAVGGCMAQLDRKKIADT APWVSAVFGTKNIEDLPKLLDQNRATGKAQVQVTEQLRQFPSQLPAARASRISSWVAISV GCNNTCTFCIVPTTRGKEKDRRPGDILDEIRQCVADGAKEVTLLGQNVNSFGYGIGDRYA FSKLLRACGTIDGLERVRFTSPHPAAFTDDVIAAMAETPNIMHQLHFPLQSGSDRILRAM RRSYRSAKFLDILGRIRAAMPDAQISTDIIVGFPGETEEDFQQTMDVVRQARFSSAFTFI YSPRPGTPAAAMEQIPRDVVQDRFDRLVALQEQITEENLATFEGRDVEVMITGKLGKKDT DTHRVTGREKTGVLVHIGVPEGEPVPEIGDFVTATVTHAGRHNLLADPDVAAGQTYSVRH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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