Request QuoteCatalog Number: xP815752DSTSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP815752DSTYeast1mgQuote
EP815752DSTE. coli1mgQuote
BP815752DSTBaculovirus200ugQuote
MP815752DSTMammalian Cell200ugQuote

Protein Information

SpeciesChlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
UniProt IDQ8KB05
Gene NamemiaB; Locus:CT1993
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMRQRMSRMPSSFYIHTFGCQMNQADSEIVTALLRAEGFVPSADETNADIVLLNSCAVREN AEERLGNILMHLKGRKRRCKELVIGVLGCVPQFERERVFSDYPFVDFIVGPDNYRELAGL VAGLREAVARPALLDYDQTETYAGIEPVRAGSISAFLPVMRGCNNHCAFCVVPVTRGRER SVGFERVVAEVVALEKAGFREVTLLGQNVNSWRDAEKGLDFAGLLEGVSLAVPSMRIRFT TSHPKDISEALVKVIAARPNLCNHIHLPVQSGSSRMLDLMKRGHTREEYLDRIAMIRSYI PEVAITTDLIAGFCTETEKDHRETLSLMEAVGYDTAFMFHYSVRPGTWAARNLPDDVPDT VKKARLQEIIELQNAISREIFQREIGKTVEVLAEAESKRSESMLMGRTPENRVVVFSRGR FNPSDTLLVKITGATSATLSGEAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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