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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP804142BMZYeast1mgQuote
EP804142BMZE. coli1mgQuote
BP804142BMZBaculovirus200ugQuote
MP804142BMZMammalian Cell200ugQuote

Protein Information

SpeciesBuchnera aphidicola subsp. Baizongia pistaciae (strain Bp)
UniProt IDQ89AC2
Gene NamemiaB; Locus:bbp_392
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMKNNKIYIKTWGCQMNEYDSALITQILKQKHGYENTKDPKLANVLILNTCSIREKAQEKV FHQLGRWKKLKQKNPNLIIAVGGCVATQEGKNIYKRANYVDIIFGTQTLHYLPNMIQEVK KNKKSVTNIDFPLTEKFNFIEYPRKPKVTAFVSIMEGCNKFCSFCIVPYTRGHEISRPVD DILLEISTLSSRGVKEIHLLGQNVNSYKGKTFNGDICKFSNLLRLVASIDGIDRIRFTTS NPFEFTDDIIEIYAEIPKIVSFLHLPVQSGSNRILQLMKRMHTIDEYKTIINKILKLRPN IQISSDFIIGFPGETLIDFEQTLQLIKDLNIDMSYSFIYSPRPGTPASELQDNVTLCEKQ KRLHILQTLIRNNTTMWNQKMLGSIQSVLVEGRSQKNPKELFGRTENNRIVNFKGNQNMI GEFINLKITKINPNSLRGSYEKRNN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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