Request QuoteCatalog Number: xP804570CMNSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP804570CMNYeast1mgQuote
EP804570CMNE. coli1mgQuote
BP804570CMNBaculovirus200ugQuote
MP804570CMNMammalian Cell200ugQuote

Protein Information

SpeciesClostridium tetani
UniProt IDQ895H1
Gene NamemiaB; Locus:CTC_01303
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-453
Expression Tag6xHis
Purity>90%
AA SequenceMLNNIVNTIHSDKNQKGTFFIETWGCQMNEEDSEKLSGMLKNIGYKNAEDKNQADIIIFN TCCVRENAELKVYGNLGALKGLKSKNPNLIIAVCGCMMQQEGMAEAIIKKYPFVDIIFGT HNSYKFPEYLNRAKQEGKSIIEVWDKEEEIVEGIPVDRKSSTKAFVTIMYGCNNFCTYCI VPYVRGRERSREVSDIEKEIKELVKSGYKEITLLGQNVNSYGKDLEPKVSFAELLRHVNE IEGIERVRFMTSHPKDLTEDVIYAIKECDKLCNHIHLPVQSGSSRILKKMNRYYNREDYL NLVNKIKEEIPNVAITTDIIVGFPGETEEDFNETLELVKEVEYDSAFTFLYSKRKGTPAY DIEEQVPEDVKHDRFKKLVEVVNKSCEKNNKKYQDRIVKVLVEGESKNDKNKLSGRTDTA KLVNFIGNKDNIGKIVDVKITKTLSFSLEGEEV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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