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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP806750BDUYeast1mgQuote
EP806750BDUE. coli1mgQuote
BP806750BDUBaculovirus200ugQuote
MP806750BDUMammalian Cell200ugQuote

Protein Information

SpeciesBacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
UniProt IDQ8A2W0
Gene NamemiaB; Locus:BT_3195
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-455
Expression Tag6xHis
Purity>90%
AA SequenceMNELTGADFKSATADDNKKLFIETYGCQMNVADSEVIASVMQMAGYSVAETLEEADAVFM NTCSIRDNAEQKILNRLEFFHSLKKKKKALIVGVLGCMAERVKDDLITNHHVDLVVGPDA YLTLPELIAAVEAGEKAINVDLSTTETYRDVIPSRICGNHISGFVSIMRGCNNFCTYCIV PYTRGRERSRDVESILNEVADLVAKGYKEVTLLGQNVNSYRFEKPTGEVVTFPMLLRMVA EAAPGVRIRFTTSHPKDMSDETLEVIAQVPNVCKHIHLPVQSGSSRILKLMNRKYTREWY LDRVAAIKRIIPDCGLTTDIFSGFHSETEEDHQLSLSLMEECGYDAAFMFKYSERPGTYA SKHLEDNVPEDVKVRRLNEIIALQNRLSAESNQRCIGKTYEVLVEGVSKRSRDQLFGRTE QNRVVVFDRGTHRIGDFVNVRVTEASSATLKGEEV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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