Request QuoteCatalog Number: xP803757BVWSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP803757BVWYeast1mgQuote
EP803757BVWE. coli1mgQuote
BP803757BVWBaculovirus200ugQuote
MP803757BVWMammalian Cell200ugQuote

Protein Information

SpeciesBradyrhizobium japonicum (strain USDA 110)
UniProt IDQ89W97
Gene NamemiaB; Locus:bll0795
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-465
Expression Tag6xHis
Purity>90%
AA SequenceMTPPRKLHIKSYGCQMNVYDAQRMVDTLAPEGFVETASAEDADLVILNTCHIREKASEKV YSELGRLRVAKDEAARGGRAMQIAVAGCVAQAEGEEIVRRAPVVDVVVGPQSYHHLPELL KRAGNEGRAVETEFPAADKFGFLAQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG AEVSRPVARIVDDVKRLADNGVRELTLIGQNVNAYHGDGPDGKSWPLGRLLEHLAGIPGI ARLRYSTSHPRDVDDSLIAAHRDLAALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVID RFRAARQDIAFSSDFIVGFPGESEQDFLATLALVTQIGYAAAYSFKYSARPGTPAADMQE TVSPAEMDQRLERLQELIDSQQSAFNKAAIGSTVDVLFERPARKDGQIVGRTAFLQPAHV MASPGIIGQILPVRIDSLERYSFLGELVTPRVAREPALSPIATGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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