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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP763352EYQYeast1mgQuote
EP763352EYQE. coli1mgQuote
BP763352EYQBaculovirus200ugQuote
MP763352EYQMammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4)
UniProt IDQ7V0F7
Gene NamemiaB; Locus:PMM1305
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-464
Expression Tag6xHis
Purity>90%
AA SequenceMLTKPIQEEEKIYKNSSLGSYWITTFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVL YNTCTIRDSAQQKVYSFLGKQVKRKHSTPKLKLVVAGCLAQQEGESLLRRVPELDLIMGP QHVNNLENLLERVDLGNQVVATEETFISEDITNPRRDSSICGWVNIIYGCNERCSYCVVP SVRGKEQSRYPDAIKSEIESLAHNNFKEVTLLGQNIDAYGRDLPGTTKEGRKENSLTDLL YFIHDVEGIKRIRFSTSHPKYFSKRLINACYELDKVCEHFHIPFQSGNNEILRLMARGYT IEKYKRIIENIRQLMPNASITADAIVAFPGETETQYRETLKLISDIGFDQVMTAAYSPRP NTPAAFWDNQIPEEVKKERLKEINELVETTSRKRNQRYLNNIESVLIEGLNPKNNAQMMG RTRTNRLTFVEISKNIEFNYSYGDEINVKITEARSFSLSGQIYK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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