Request QuoteCatalog Number: xP775006VFESize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP775006VFEYeast1mgQuote
EP775006VFEE. coli1mgQuote
BP775006VFEBaculovirus200ugQuote
MP775006VFEMammalian Cell200ugQuote

Protein Information

SpeciesVibrio parahaemolyticus
UniProt IDQ87RP4
Gene NamemiaB; Locus:VP0733
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLLIKTWGCQMNEYDSSKMADLLNAANGYELTEEPEEADVLLLNTCSIREKAQEKVF HQLGRWKTLKDKKPGVVIGVGGCVATQEGDHIRERAPYVDVIFGPQTLHRLPEMIKQSQT DDAPVMDISFPEIEKFDRLPEPRAEGATAFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDD VLFEIAQLAEQGVREVNLLGQNVNAYRGPMHDGEICSFAELLRLVASIDGIDRIRFTTSH PLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI QISSDFIVGFPGETDKDFQDTMKLIKDVDFDMSFSFIFSPRPGTPAADYPCDIPEQVKKE RLYELQQTINAQAMRYSRLMLATEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIG QFVDVKITDVFANSLRGELVRTEKDMDLRSVISPTQMMAKTRREDELGVATFTP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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