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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP733317Yeast1mgQuote
EP733317E. coli1mgQuote
BP733317Baculovirus200ugQuote
MP733317Mammalian Cell200ugQuote

Protein Information

SpeciesLegionella pneumophila (strain Paris)
UniProt IDQ5X5N2
Gene NamemiaB; Locus:lpp1288
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMVKKLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVF SQLGQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLE KNKSVVDISFPEIEKFDHLPAPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEISRPFDD VLAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSH PLAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDI RLSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQ RLQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQLSGRTECNRVVNFDGPPHLIG QFIDVQISDALPNSLRGRLLEKEMQPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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