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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP724860Yeast1mgQuote
EP724860E. coli1mgQuote
BP724860Baculovirus200ugQuote
MP724860Mammalian Cell200ugQuote

Protein Information

SpeciesErwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum)
UniProt IDQ6D7K6
Gene NamemiaB; Locus:ECA1319
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLGSTHGYELTEIAEEADVLLLNTCSIREKAQEKVF HQLGRWKALKDLNPNLIIGVGGCVASQEGAHIRERAHYVDVIFGPQTLHRLPEMINHVQG TRSPIVDISFPEIEKFDRLPEPRADGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPCDD VLFEIAQLAAQGVREVNLLGQNVNAYRGETYDGEICSFAELLRLVAAIDGIDRVRFTTSH PIEFTDDIISVYEDTPELVSFLHLPVQSGSDRVLTMMKRRHTALEYKAIIRKLHNARPGI LISSDFIIGFPGETQADFEQTMKLIADVNFDMSYSFVYSARPGTPAADMVDDVLEEEKKQ RLYLLQDRITKQAMRFSRLMLGTVQRILVEGTSRKSVMELSGRTENNRVVNFEGTPDMIG KFVDVEIVDVYTNSLRGIVVRTEDQMDLRVHESPSSVIARTRKENEIGVGFYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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