Request QuoteCatalog Number: xP740609CQFSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP740609CQFYeast1mgQuote
EP740609CQFE. coli1mgQuote
BP740609CQFBaculovirus200ugQuote
MP740609CQFMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium diphtheriae
UniProt IDQ6NGR0
Gene NamemiaB; Locus:DIP1448
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-516
Expression Tag6xHis
Purity>90%
AA SequenceMFNDIESHRVPNVGQGRSFEVRTFGCQMNVHDSERLSGLLEEAGYHAVADGEEPDLVVFN TCAVRENADKRLYGTLGQLRSAKEKNPRMQIAVGGCLAQKDKDTVVAKAPWVDAVFGTHN MGALPSLLSRSEHNKRAEVEIVDSLEQFPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVP SLRGKEVDRRPGDILAEVQALVDQGVSEVTLLGQNVNAYGVNFSDPDIQRDRFAFSKLLR ACGKIEGLERLRFTSPHPAEFTHDVIDAMAETPNVCPQLHMPLQSGSDKVLKEMRRSYRS KKFLGILEEVRAKIPHASITTDIIVGFPGETEEDFQETLNVVEKARFTSAYTFQYSPRPG TPAADYADQVPKEVVQDRYERLLALQERISTEENAKLIGTEVELLVQASGGRKNDKTQRM TGRSRDGRLVHFDPQGHVDGDIRPGDVITTVVTEAKPFFLIADSGVLQHRRTKAGDMSAA GKVPTTAPVGVGLGLPSIGSPAQKRSETSKSSGCGC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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