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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP750676RLUYeast1mgQuote
EP750676RLUE. coli1mgQuote
BP750676RLUBaculovirus200ugQuote
MP750676RLUMammalian Cell200ugQuote

Protein Information

SpeciesRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
UniProt IDQ6NCM4
Gene NamemiaB; Locus:RPA0448
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-463
Expression Tag6xHis
Purity>90%
AA SequenceMSPPRKLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKV YSELGRLRVARDEAANHGRRMQIAVAGCVAQAEGAEIIRRAPVVDVVVGPQSYHNLPQLL AKAEQHGRALETEFPIEDKFGVLPQPAPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRG AEMSRPVAAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDRLAWSLGRLVRRLAEIPGI VRLRYSTSHPNDVNDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVID RFRLASEAIAFSSDFIVGFPGETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMAE MVPAAVMDERLEQLQQLIDQQQSAFNKAAIGRTVEVLFERAGRKPGQIVGRTAYLQPAHV MAPDSIIGKVLPVRVDSLERYSLLGELASATSRPADAMAATGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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