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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP750613BFPYeast1mgQuote
EP750613BFPE. coli1mgQuote
BP750613BFPBaculovirus200ugQuote
MP750613BFPMammalian Cell200ugQuote

Protein Information

SpeciesBdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
UniProt IDQ6MLC6
Gene NamemiaB; Locus:Bd2086
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-453
Expression Tag6xHis
Purity>90%
AA SequenceMDDITQNLESVEKNPDIGMGRGVYISTYGCQMNVNDTERMYALLEMQNFVPVTDPKKASL IIINSCSVREKPVHKVYSEVGTYKYMKRKNPELKIGVGGCVGQQEKENLMKTQPMIDFVF GTDQIDSLPQLVAKSFAGERRLVNSRFEHRSPYHIETLVRNPGVATYVNITKGCDNFCTF CVVPYTRGREKSRPVQHILTDIRHLVKRGVKEVTLLGQNVNSYQGDEGIDFADLLAKVAK ETDVERIRYTTSHPKDFNQKLVDVMAEHSNKIMEYIHLPFQAGSTKVLERMNRNYTREEY LERIAMIQKGLPNVCFSTDIIVGFPGETEEDFQDTLNMVTEVGFETIFAFSYSPRPFTKA AKFEDQLPEDVKNERLNRLFDVHEAMAFERVKRYEGTTMKVLVENVDRDHGKMQGRSTGN KLVHFLGTADLIGKTVDVKITKAFPAVFRGEMI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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